Part:BBa_K3096040

Temperature responsive repression system

A constitutively active promoter expresses Clts, a temperature sensitive cI repressor of the lambda phage. If the temperature is significantly below 37°C (body temperature), Clts is an active repressor for the cI lambda promoter. This promoter controls the transcription of a repressor protein, AraC. Therefore, if conditions are not 37°C, the AraC is not available, hence the pBAD promoter, controlled by AraC, is active and genes controlled by the pBAD promoter are transcribed. Accordingly, if the temperature is at 37°C, Clts is unstable and cannot repress the expression of AraC. AraC consequently inhibits the pBAD promoter and downstream genes are not expressed.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  Illegal NotI site found at 781
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]